2D-DIGE for quantitative proteomics (CAT#: STEM-MB-0241-WXH)

Introduction

Two-dimensional fluorescence difference gel electrophoresis(2D-DIGE ), is a new quantitative technology for proteomics developed on the basis of traditional two-dimensional electrophoresis. The principle of 2D-DIGE to separate mixed proteins is consistent with that of two-dimensional electrophoresis. The difference in isoelectric point and molecular weight of proteins is used to separate protein mixtures. At the same time, the sensitivity of fluorescent dyes and internal standards are used to make it suitable for quantitative proteomics research. The effect is obviously better than the traditional two-dimensional electrophoresis. The fluorescent dyes used by DIGE are Cy2, Cy3 and Cy5, which can react with the lysine side chain amino group of the protein to label the protein. The isoelectric point and molecular weight of the labeled protein are not affected, and the labeled protein is mixed in equal amounts. After two-dimensional electrophoresis, different gels can be matched by the cy2 internal standard to eliminate the differences between the gels, and the changes in protein expression are reflected by the different fluorescence intensities of cy3 and cy5.