AFLP PCR (CAT#: STEM-MB-0197-WXH)

AFLP PCR uses restriction enzymes to digest genomic DNA and allows attachment of adaptors to the sticky ends of the fragments. A part of the restriction fragments is then selected to be amplified by using primers that are complementary to the adaptor sequence. The amplified sequences are separated and visualized on denaturing on agarose gel electrophoresis.

• Assess genetic diversity within species or among closely related species.
• Infer population-level phylogenies and biogeographic pattern.
• Generate genetic maps.
• Determine relatedness among cultivars.

1.Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments.
2.Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences.
3.Electrophoretic separation of amplicons on a gel matrix, followed by visualisation of the band pattern.