Alu PCR (CAT#: STEM-MB-0199-WXH)

Introduction

Alu elements are non-autonomous retrotransposons present in high number of copies in primate genomes. In the human genome, these repetitive sequences are interspersed on all chromosomes with more than one million copies. Thanks to their genome-wide distribution, Alu elements can be used as target loci to perform a PCR-based genome fingerprinting.
The method is characterised by an high information level compared to other DNA fingerprint techniques.

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Principle Applications Procedure Materials Notes Related Products

Principle

Alu PCR is a rapid and easy DNA fingerprinting technique based on the simultaneous analysis of many genomic loci surrounded by Alu repetitive elements.

Applications

• Detection of genetic polymorphisms and mutations in human and primate genomes.
• Human DNA quantitation in forensic.

Procedure

1.Isolation of DNA
2.PCR amplification
3.Separation of PCR reactions by electrophoresis

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