Analysis Affinity and Kinetics of 2,4,6-Trinitrotoluene (TNT) by KinExA (CAT#: STEM-MB-0061-CJ)

Introduction

2,4, 6-trinitrotoluene (TNT) , one of the most common explosives, is still widely used in military and industrial applications. The water, which turns pink after being contaminated with TNT and RDX, is difficult and expensive to clean up. And long-term exposure to trinitrotoluene increases the chance of anemia and abnormal liver function. Animals injected or inhaled trinitrotoluene have also been found to affect the blood and liver, spleen and other adverse effects related to the immune system. In addition, TNT has adverse effects on male reproductive function and is listed as a possible carcinogen. Ingestion of TNT will darken the urine. Can cause subacute poisoning, chronic poisoning, cataracts, toxic hepatitis, but also damage the hematopoietic system.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

KinExA is a two-stage analysis system. In the first stage, a number of solutions are prepared, where one partner remains constant (constant binding partner, or CBP) and the other (titrant) is variable, usually serially diluted. As the titrant is added, the free CBP decreases and is analysed by a sophisticated and precise microfluorescence measurement device. The signal generated can be mathematically related to the affinity (KD) of the two molecules for each other, as well as the kinetic parameters of binding (kon) and dissociation (koff).

Applications

Immunology/Inflammation, Toxicology

Procedure

1. preparation of the functionalized beads which will capture the analyte for measuremen.
2. preparation of a series of solutions consisting of a constant initial concentration of one component of the binary reaction and serial dilutions of the other reactant. The component that is kept constant is the constant binding partner (CBP) , and is the one which will be analyzed.
3. each reaction mixture is sampled and the fluorescence of free CBP bound to the capture beads is obtained for subsequent numerical analysis.

Materials

• Sample Type: Lipids, Viruses, Serum
• Equipment: Kinetic Exclusion Assay (KinExA)

Other recommended products

Analysis Immunoassay of Polychlorinated Biphenyls (PCBs) by KinExA (CAT#: STEM-MB-0080-CJ)
Analysis Immunoassay of 2,4-Dinitrophenol (DNP) by KinExA (CAT#: STEM-MB-0084-CJ)
Analysis Affinity of 39F7 to Human β-Klotho by KinExA (CAT#: STEM-MB-0047-CJ)
Analysis Affinity and Kinetics of IFN-α2A with Sifalimumab by KinExA (CAT#: STEM-MB-0054-CJ)
Analysis Affinity of Anti-PD-1 by KinExA (CAT#: STEM-MB-0023-CJ)
Analysis Affinity of Clinical Anti-drug Antibody (ADA) by KinExA (CAT#: STEM-MB-0021-CJ)
Analysis Affinity and Kinetics of Prostate Specific Antigen (PSA) with Antibody M612166 by KinExA (CAT#: STEM-MB-0060-CJ)
Analysis Affinity of AMG 139 to IL-23 by Kinetic (CAT#: STEM-MB-0003-CJ)