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The extracellular matrix (ECM) consists of numerous macromolecules classified traditionally into collagens, elastin, and microfibrillar proteins, proteoglycans including hyaluronan, and noncollagenous glycoproteins. The extracellular matrix helps cells attach to, and communicate with, nearby cells, and plays an important role in cell growth, cell movement, and other cell functions. The extracellular matrix is also involved in repairing damaged tissue.The protease and ECM binding properties of HtrA from L. monocytogenes (LmHtrA) can be studied using recombinant proteins such as full length His-tagged rLmHtrA wild type (rLmHtrA1-501). And Full length His-tagged can active site mutant S343A (rLmHtrA1-501(S343A)) and His-tagged ∆PDZ (rLmHtrA1-394∆PDZ).