Analysis Immunoassay of Neonicotinoid Insecticide Thiamethoxam by KinExA (CAT#: STEM-MB-0073-CJ)

Introduction

Neonicotinoid insecticide Thiamethoxam belongs to 1,3,5-oxadiazines derivatives. And neonicotinoids are synthetic compounds similar in structure to nicotine. They have a common mode of action (binding to nicotinic acetylcholine receptors) that affects the central nervous system of insects, making them active against a broad spectrum of insects. It is also an endogenic insecticide that can be absorbed through the roots of plants and transferred to leaves, flowers and pollen, making it an ideal candidate for seed coating.

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Principle Applications Procedure Materials Notes Related Products

Principle

KinExA is a two-stage analysis system. In the first stage, a number of solutions are prepared, where one partner remains constant (constant binding partner, or CBP) and the other (titrant) is variable, usually serially diluted. As the titrant is added, the free CBP decreases and is analysed by a sophisticated and precise microfluorescence measurement device. The signal generated can be mathematically related to the affinity (KD) of the two molecules for each other, as well as the kinetic parameters of binding (kon) and dissociation (koff).

Applications

Immunology/Inflammation, Toxicology

Procedure

1. preparation of the functionalized beads which will capture the analyte for measuremen.
2. preparation of a series of solutions consisting of a constant initial concentration of one component of the binary reaction and serial dilutions of the other reactant. The component that is kept constant is the constant binding partner (CBP) , and is the one which will be analyzed.
3. each reaction mixture is sampled and the fluorescence of free CBP bound to the capture beads is obtained for subsequent numerical analysis.

Materials

• Sample Type: Serum, Plasma, Urine
• Equipment: Kinetic Exclusion Assay (KinExA)

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