Analysis Interactions of Flavodoxin Reductases (FNRs) and Flavodoxin-Like Protein (NrdI) by Microscale Thermophoresis (MST) (CAT#: STEM-MB-2447-LGZ)

Introduction

Microscale Thermophoresis (MST) is the directed movement of particles in a microscopic temperature gradient. Affinity is determined by measuring trace thermal changes resulting from changes in the hydration layer (usually caused by changes in the structure/conformation of biomolecules). Even small changes like protein phosphorylation or small molecules binding to a target can be measured. MST also allows measurement of intermolecular interactions directly in solution without the need for a fixed surface (no fixation required).<br />NrdI is essential in the activation of the manganese-bound form of the class Ib ribonucleotide reductase (RNR) system. RNRs, in turn, are the only source of the de novo synthesis of deoxyribonucleotides required for DNA replication and repair in all living organisms