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Analysis Kinetics of Anti-Müllerian Hormone (AMH) by BLI (CAT#: STEM-MB-0254-CJ)

Introduction

Anti-Müllerian hormone (AMH) is a 140-kDa dimeric glycoprotein that is commonly used as a biochemical marker for the assessment of the ovarian reserve and female fertility. The measurement of the concentration of AMH is relevant for assessing the success or failure of assisted reproductive therapies and to support the diagnosis of some conditions such as polycystic ovary syndrome, the neonatal gender determination, and the evaluation of testicular function and cryptorchidism. Serum and plasma AMH levels are relatively stable even under the use of hormonal contraceptives or during spontaneous menstrual cycle.In immunoassays, antibodies play a key role in the detection and presentation of antigens for diagnosis.




Principle

Bio-Layer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. BLI experiments are used to determine the kinetics and affinity of molecular interactions. In a BLI experiment, one molecule is immobilized to a Dip and Read Biosensor and binding to a second molecule is measured. A change in the number of molecules bound to the end of the biosensor tip causes a shift in the interference pattern that is measured in real-time.

Applications

Oncology & Cancer; Pharmacology

Procedure

1. Detect Buffers and prepare samples. BLI experiments are set up with one molecule immobilised on the surface of the biosensor (load sample) and a second molecule in solution (the analytical sample).
2. Fix the load sample on the biocompatible biosensor while the analytical sample is in solution.
3. The biosensor tip is immersed in the solution so that the target molecule begins to bind to the analysis sample.
4. Set up and run the BLI experiment. Molecules bound to or dissociated from the biosensor can generate response curves on the BLI system; unbound molecules, changes in the refractive index of the surrounding medium or changes in flow rate do not affect the interferogram pattern.
5. Collect and analyse data on the BLI's system.

Materials

• Equipment: Gator® Bio-Layer Interferometry (BLI)
• Sample Type: DNA, RNA, Protein, Antibodies, Peptides, Small Molecules
• Optionals: Electroporator