Analysis Kinetics of Biowarfare Agents by KinExA (CAT#: STEM-MB-0262-CJ)

Introduction

Biological Warfare agents are microorganisms like virus, bacteria, fungi, protozoa or toxins produced by them, that give rise to diseases in man, animals or plants, when deliberately dispersed in an area. These agents can cause large-scale mortality, morbidity and can incapacitate a large number of people in the shortest possible time and have adverse effects on human health. The use of biological warfare (BW) agents can be covert or overt and they differ from conventional weapons by way of several unique properties. The effects of these agents are not instantaneous and require few hours to weeks before the symptoms appear in the affected population. These attacks require a release of small quantity of viable material and are capable of self-replication and can cause a disease outbreak in an area.

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Principle Applications Procedure Materials Notes Related Products

Principle

Bio-Layer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. BLI experiments are used to determine the kinetics and affinity of molecular interactions. In a BLI experiment, one molecule is immobilized to a Dip and Read Biosensor and binding to a second molecule is measured. A change in the number of molecules bound to the end of the biosensor tip causes a shift in the interference pattern that is measured in real-time.

Applications

Immunology/Inflammation, Toxicology

Procedure

1. Detect Buffers and prepare samples. BLI experiments are set up with one molecule immobilised on the surface of the biosensor (load sample) and a second molecule in solution (the analytical sample).
2. Fix the load sample on the biocompatible biosensor while the analytical sample is in solution.
3. The biosensor tip is immersed in the solution so that the target molecule begins to bind to the analysis sample.
4. Set up and run the BLI experiment. Molecules bound to or dissociated from the biosensor can generate response curves on the BLI system; unbound molecules, changes in the refractive index of the surrounding medium or changes in flow rate do not affect the interferogram pattern.
5. Collect and analyse data on the BLI's system.

Materials

• Equipment: ForteBio Bio-Layer Interferometry (BLI)
• Sample Type: DNA, RNA, Protein, Antibodies, Peptides, Small Molecules

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