Analysis Kinetics of T. vaginalis AP65_A1 Aptamer by BLI (CAT#: STEM-MB-0274-CJ)

Introduction

In antigen-based immunoassays, cells surface proteins are potentially good candidates as target molecules for detection of pathogen cells. The adhesion protein 65 (AP65) of T. vaginalis is a prominent adhesin that is located on the parasite’s cell surface and is secreted to the extracellular environment. It plays a role in the adhesion of the parasite to host cell 22 and to iron-rich heme and hemoglobin 2324. AP65 is believed to be a unique protein of T. vaginalis have shown no crosshybridization and immuno-crossreactivity of AP65 to other trichomonads found primarily in animals, e.g. Trichomonas suis, Pentatrichomonas hominis, and Tritrichomonas foetus.

Aptamers are oligonucleotides, either single or double-stranded deoxyribonucleic acids (DNAs) or ribonucleic acids (RNAs) that can bind to a variety of molecules with high affinity and specificity, exhibiting several properties that make them interesting as an alternative to antibodies as tools for analytical applications. Aptamers, being inherently nucleic acid in nature are far more flexible, stable and cost-effective as compared to antibodies. Aptamers are selected using an in vitro process.