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Analysis of A-GAMMA3'E Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2202-LGZ)

Introduction

Official Full Name: A-gamma-globin 3' regulatory region<br />Also known as: A-gamma-e<br />This genomic region represents the regulatory element downstream of the hemoglobin subunit gamma 1 (HBG1) gene, also known as the a-gamma gene in the beta-globin gene cluster on chromosome 11. Two elements are defined in this region, an enhancer called Enh and a silencer called f. The enhancer was active in transfection assays in both erythroid leukemia and non-erythroid cell lines, and it may include both positive and negative acting components. Both the Enh and F elements can negatively regulate transcription at the beta-globin locus in an embryonic-specific manner in some transgenic contexts, though they are not required for developmental switching from fetal gamma-globin to adult beta-globin gene expression in transgenic m the ice . These elements form a hypersensitive region of erythroid DNase I with multiple subsites. This region binds a variety of proteins, including the matrix-associated factor SATB1, the HOXB2 transcription factor, and the erythroid GATA-1-binding protein. Mutations in this region may result in increased gamma-globin gene expression. These elements are deleted in certain β-thalassemias, along with deletion of the downstream adult β-globin gene.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements