Analysis of ABCA2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2015-LGZ)

Introduction

Official Full Name: ATP binding cassette subfamily A member 2
Also known as: ABC2; IDPOGSA
The membrane-associated protein encoded by this gene is a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules outside and inside the cell membrane. The ABC genes are grouped into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found only in multicellular eukaryotes. The protein is highly expressed in brain tissue and may play a role in macrophage lipid metabolism and neurodevelopment. Two transcript variants of this gene have been found to encode different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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