Analysis of ABCG5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1723-LGZ)

Introduction

Official Full Name: ATP binding cassette subfamily G member 5
Also known as: STSL; STSL2
The protein encoded by this gene is a member of the ATP-binding cassette transporter superfamily. ABC proteins transport various molecules outside and inside the cell membrane. The ABC genes are grouped into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the albumin subfamily. The protein encoded by this gene acts as a hemi-transporter, limiting intestinal absorption and promoting biliary excretion of sterols. It is expressed in the liver, colon and intestine in a tissue-specific manner. The gene is arranged in tandem on chromosome 2, in a head-to-head arrangement with the family member ABCG8. Mutations in this gene may lead to sterol accumulation and atherosclerosis and have been observed in patients with sitosterolemia.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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