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Analysis of ACACA Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2489-LGZ)

Introduction

Official Full Name: acetyl-CoA carboxylase alpha<br />Also known as: ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha<br />Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two forms of ACC, alpha and beta, encoded by two different genes. ACC-α is highly enriched in adipogenesis tissues. The enzyme is controlled long-term at the transcriptional and translational levels and regulated in the short-term by phosphorylation/dephosphorylation of targeted serine residues and allosteric conversion of citrate or palmitoyl-CoA. Multiple different alternatively spliced transcript variants were found in the 5' sequence of this gene, encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements