Analysis of Acetylcholinesterase (AchE) by Enzyme-labeled Instrument (CAT#: STEM-MB-1409-LGZ)

Introduction

Sensitivity: 1.225 U/mL
Detection range: 1.225-490 U/mL
Precision: inter-lot difference of 9.300000000000001%, intra-lot difference of 4.7%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 412 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Acetylcholinesterase (AchE) catalyzes the hydrolysis of acetylcholine (Ach) to choline, and the reaction of choline with disulfide p-nitrobenzoic acid (DTNB) to produce 5-merhydryl-nitrobenzoic acid (TNB). TNB had a characteristic absorption peak at 412 nm. By measuring the absorbance increase rate at 412 nm, the activity of AchE was calculated.

Applications

It is suitable for the detection of acetylcholinesterase activity in serum, plasma and animal tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma and animal tissue samples

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