Analysis of Acholeplasma Laidlawii by Real-Time PCR Method (CAT#: STEM-MB-3757-LGZ)

Introduction

Acholeplasma laidlawii are mycoplasma, prokaryotes with a single cell membrane, the plasma membrane. There are no intracellular membranes and no cell wall. Unlike other bacteria, mycoplasma have no inner membrane and they do not synthesize cholesterol. These organisms are among the simplest self-replicating cells known.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.