Analysis of Actinomyces Gerencseriae by Real-Time PCR Method (CAT#: STEM-MB-3016-LGZ)

Introduction

Actinomyces gerencseriae is a nonmotile, Gram positive bacterial species. The average Actinomyces gerencseriae colony is around 0.2 mm in diameter, circular in shape, are branching filamentous and white in color. Actinomyces gerencseriae have an incubation period of 2 days and is a non-spore forming bacterial species.
A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.