Analysis of ADAM15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0774-LGZ)

Introduction

Official Full Name: ADAM metallopeptidase domain 15
Also known as: MDC15
The protein encoded by this gene is a member of the ADAM (disintegrin and metalloprotease) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. The protein contains multiple functional domains, including a zinc-binding metalloprotease domain, a disintegrant-like domain, and an egf-like domain. Through its disintegrant-like domain, this protein specifically interacts with the integrin chain β3. It also interacts with Src family protein tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may play a role in cell-cell adhesion as well as cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been observed.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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