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Analysis of ADAMTS4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0773-LGZ)

Introduction

Official Full Name: ADAM metallopeptidase with thrombospondin type 1 motif 4<br />Also known as: ADMP-1; ADAMTS-2; ADAMTS-4<br />This gene encodes a member of the ADAMTS (a disintegrating and metalloprotease with thrombospondin motif) protein family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family vary in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks the c-terminal TS motif. The encoded preproprotein undergoes proteolytic processing to generate the mature protease. This protease is responsible for degrading cartilage's major proteoglycan aggregate protein and the brain-specific extracellular matrix protein brevican. Expression of this gene is upregulated in arthritic disease, which may contribute to disease progression through degradation of aggregated proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes the proteolytically processed isoform.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements