Analysis of AGPAT2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2020-LGZ)

Introduction

Official Full Name: 1-acylglycerol-3-phosphate O-acyltransferase 2<br />Also known as: BSCL; BSCL1; LPAAB; LPLAT2; 1-AGPAT2; LPAAT-beta<br />This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. Located within the membrane of the endoplasmic reticulum, this protein converts lysophosphatidic acid to phosphatidic acid, the second step in phospholipid biosynthesis. Mutations in this gene are associated with either congenital generalized lipodystrophy (CGL) or Berardinelli-Seip syndrome, a disorder characterized by near loss of fat tissue and severe insulin resistance. Alternative transcriptional splice variants encoding different isoforms have been characterized.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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