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Analysis of AGT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0766-LGZ)

Introduction

Official Full Name: angiotensinogen<br />Also known as: ANHU; hFLT1; SERPINA8<br />The protein encoded by this gene, angiotensinogen precursor or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin when blood pressure decreases. The resulting product, angiotensin I, is cleaved by angiotensin-converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. This protein is involved in the maintenance of blood pressure, fluid and electrolyte homeostasis, and is involved in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension and can lead to tubular dysplasia, a serious disorder of renal tubular development. Defects in this gene have also been associated with nonfamilial forms of structural atrial fibrillation and inflammatory bowel disease.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements