Analysis of AGTRAP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1271-LGZ)

Introduction

Official Full Name: angiotensin II receptor associated protein
Also known as: ATRAP
This gene encodes a transmembrane protein localized to the plasma membrane and perinuclear vesicle structures. The gene product interacts with the angiotensin II type receptor and negatively regulates angiotensin II signaling. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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