Analysis of AGXT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1556-LGZ)

Introduction

Official Full Name: alanine--glyoxylate aminotransferase
Also known as: AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1; Ser-PyrAT
This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of MIR4299 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2421-LGZ)
Analysis of SLC30A6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1133-LGZ)
Analysis of Schistosoma Incognitum by Real-Time PCR Method (CAT#: STEM-MB-3361-LGZ)
Analysis of Pseudomonas Mallei by Real-Time PCR Method (CAT#: STEM-MB-3158-LGZ)
Analysis of Ovine Adenovirus by Real-Time PCR Method (CAT#: STEM-MB-3026-LGZ)
Analysis of Dictyocaulus Arnfieldi by Real-Time PCR Method (CAT#: STEM-MB-2582-LGZ)
Analysis of Myxosporidia Spp. by Real-Time PCR Method (CAT#: STEM-MB-3776-LGZ)
Analysis of BTG2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0235-LGZ)