Analysis of Aiolos (Human) by ELISA (CAT#: STEM-MB-0653-LGZ)

Introduction

The Ikaros family has a C2H2 zinc finger structure (CX2-3-C-X3-Phe-XS-L-X2-H-X3-H), which is essential for the early genesis, development and normal function of lymphocytes and other hematopoietic cells.
Aiolos belongs to the zinc finger family of Ikaros transcription factors and is expressed by various immune cells. Aiolos can form homologous or heterodimer with Ikaros, or interact with other non-Ikaros zinc finger family transcription factors and epigenetic regulators to regulate gene expression through direct binding of its zinc finger motifs to target DNA.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: serum, plasma, tissue homogenates, cell lysates

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