Analysis of AKNAD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1178-LGZ)

Introduction

Official Full Name: AKNA domain containing 1<br />Also known as: C1orf62<br />This gene encodes a protein which contains a domain found in an AT-hook-containing transcription factor. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Bifidobacterium Spp. by Real-Time PCR Method (CAT#: STEM-MB-3417-LGZ)
Analysis of Human Immunodeficiency Virus 1 (HIV-1O) by Real-Time PCR Method (CAT#: STEM-MB-3219-LGZ)
Analysis of Raillietina Spp. by Real-Time PCR Method (CAT#: STEM-MB-4052-LGZ)
Analysis of NIBAN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0487-LGZ)
Analysis of Strongyloides Avium by Real-Time PCR Method (CAT#: STEM-MB-3014-LGZ)
Analysis of Human Rhinovirus 9 by Real-Time PCR Method (CAT#: STEM-MB-3269-LGZ)
Analysis of UCHL5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0739-LGZ)
Analysis of KCNA10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1205-LGZ)