Analysis of ALDOB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1933-LGZ)

Introduction

Official Full Name: aldolase, fructose-bisphosphate B
Also known as: ALDB; ALDO2
Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate . There are 3 aldolase isozymes in vertebrates, which differ by their electrophoretic and catalytic properties. The differences suggest that aldolases A, B, and C are distinct proteins, products of a related "housekeeping" gene family that exhibit developmentally regulated expression of distinct isozymes. Developing embryos produce aldolase A, and even more so in adult muscle, which can account for up to 5% of total cellular protein. In the adult liver, kidney, and intestine, expression of aldolase A is repressed and aldolase B is produced. Aldolases A and C are expressed in roughly equal amounts in the brain and other neural tissues. Aldolase a and c have a high degree of homology. ALDOB deficiency causes hereditary fructose intolerance.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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