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Analysis of γ-Aminobutyric Acid (GABA) by Enzyme-labeled Instrument (CAT#: STEM-MB-1452-LGZ)

Introduction

Sensitivity: 0.06 μmol/mL
Detection range: 0.06–10.0 μmol/mL
Precision: inter-lot difference of 6.8%, intra-lot difference of 4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 630-650 nm




Principle

Phenol and sodium hypochlorite react with GABA to produce a blue-green product with maximum light absorption at 640 nm.

Applications

It is suitable for the determination of gamma-aminobutyric acid in animal and plant tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal and plant tissue samples
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