Analysis of Ang-like 4 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0027-LGZ)

Introduction

Angiopoietin-like proteins (ANGPTLs) are a recently discovered secretory glycoprotein family that share homology with ANG and play a role in regulating angiogenesis. Currently, it has been found that there are 8 members of the ANGPTLs family (ANGPTL1-8), and ANGPTL2, 3, and 4 affect the generation of arteriosclerosis mainly by regulating glucose and lipid metabolism and affecting angiogenesis.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma

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