Analysis of Angiotensin by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1361-LGZ)

Introduction

Sensitivity: 27.5 U/L
Detection range: 27.5 - 682 U/L
Precision: inter-lot difference of 4.4%, intra-lot difference of 3%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 340 nm

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Principle Applications Procedure Materials

Principle

N-[3-(2-furanyl) acrylyl] -L-phenylalanyl-Glycylglycine (FAPGG) had the maximum absorption peak at 340nm. ACE catalyzed the hydrolysis of FAPGG substrate to furanyl phenylalanine (FAP) and diglycine peptide (GG), which resulted in the decrease of absorbance at 340nm. ACE activity was calculated by measuring the rate of absorbance decline of FAPGG at 340 nm.

Applications

Applicable to serum (plasma), animal tissue samples of angioconvertin activity.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), animal tissue samples

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