Analysis of ANO5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2463-LGZ)

Introduction

Official Full Name: anoctamin 5
Also known as: GDD1; LGMD2L; LGMDR12; TMEM16E
This gene encodes a member of the anoctamin family of transmembrane proteins. The encoded protein is likely a calcium activated chloride channel. Mutations in this gene have been associated with gnathodiaphyseal dysplasia. Alternatively spliced transcript variants have been described.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PPP2R5A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0593-LGZ)
Analysis of Dictyocaulus Spp. by Real-Time PCR Method (CAT#: STEM-MB-2580-LGZ)
Analysis of DVL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1320-LGZ)
Analysis of Haemophilus Spp. by Real-Time PCR Method (CAT#: STEM-MB-2979-LGZ)
Analysis of EIF4E2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1763-LGZ)
Analysis of CHID1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2231-LGZ)
Analysis of PLA2G4A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0568-LGZ)
Analysis of MAMDC4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2002-LGZ)