Analysis of ANO9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2197-LGZ)

Introduction

Official Full Name: anoctamin 9
Also known as: PIG5; TP53I5; TMEM16J
The protein encoded by this gene is a member of the TMEM16 (anoctamin) family of proteins, some of which form integral membrane calcium-activated chloride channels. The function of the encoded protein has yet to be elucidated, although it may have channel-forming abilities and also may have phospholipid scramblase activity. This gene has been observed to be upregulated in stage II and III colorectal cancers.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of ACP6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0776-LGZ)
Analysis of HSD17B7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1039-LGZ)
Analysis of OR2T29 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0545-LGZ)
Analysis of Human Papillomavirus (HPV) by Real-Time PCR Method (CAT#: STEM-MB-3343-LGZ)
Analysis of Helicobacter Spp. by Real-Time PCR Method (CAT#: STEM-MB-3295-LGZ)
Analysis of TSEN15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0283-LGZ)
Quantitative Fluorescent PCR (QF-PCR) (CAT#: STEM-MB-0186-WXH)
Analysis of OR2W3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0538-LGZ)