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Analysis of AP4B1-AS1 Gene (Mutation) in Hereditary Spastic Paraplegia 47 by RT-qPCR (CAT#: STEM-MT-0126-LGZ)

Introduction

Official Symbol: AP4B1-AS1<br />Official Full Name: AP4B1 antisense RNA 1<br />Gene type: ncRNA




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary spastic paraplegia 47

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements