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Analysis of APC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1840-LGZ)

Introduction

Official Full Name: APC regulator of WNT signaling pathway<br />Also known as: GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46<br />This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant premalignant lesion that usually progresses to malignancy. Mutations in the APC gene have been found to occur in the majority of colorectal cancers, where disease-associated mutations tend to cluster in a small region known as the mutation cluster region (MCR) and lead to truncated protein products.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements