Analysis of ARID4B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0254-LGZ)

Introduction

Official Full Name: AT-rich interaction domain 4B
Also known as: BCAA; BRCAA1; RBP1L1; SAP180; RBBP1L1
The protein sequence encoded by this gene is similar to retinoblastoma binding protein-1. The encoded protein is a subunit of the histone deacetylase-dependent SIN3A transcriptional co-repressor complex that functions in a variety of cellular processes, including proliferation, differentiation, apoptosis, tumorigenesis, and cell fate determination. This gene product is recognized by IgG antibodies isolated from breast cancer patients and appears to be a molecular marker associated with a wide range of human malignancies. Alternative transcriptional splice variants encoding different isoforms have been characterized.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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