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Analysis of ASAH1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1874-LGZ)

Introduction

Official Full Name: N-acylsphingosine amidohydrolase 1<br />Also known as: AC; PHP; ASAH; PHP32; ACDase; SMAPME<br />This gene encodes a protein of the acid ceramidase family. Alternative splicing results in variants in multiple transcripts, at least one of which encodes a proprotein that is proteolytically processed. Processing of this preproprotein produces α and β subunits, which heterodimerize to form mature lysosomal enzymes that catalyze the degradation of ceramides to sphingosine and free fatty acids. This enzyme is overexpressed in a variety of human cancers and may play a role in cancer progression. Mutations in this gene have been associated with lysosomal storage disorders, Farber lipogranulomatosis, neuromuscular disorders, and spinal muscular atrophy with progressive myoclonic epilepsy.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements