Analysis of ATP by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1360-LGZ)

Introduction

Sensitivity: 0.01 mmol/L
Detection range: 0.01 mmol/L
Precision: inter-lot difference of 9.300000000000001%, intra-lot difference of 3.6%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 636 nm

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Principle Applications Procedure Materials

Principle

Creatine kinase catalyzes the reaction of creatine and ATP to produce creatine phosphate. The content of creatine phosphate is detected by colorimetric method to reflect the content of ATP.

Applications

Used to detect the amount of ATP in animal tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissue samples

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