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Analysis of ATP1B1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0779-LGZ)

Introduction

Official Full Name: ATPase Na+/K+ transporting subunit beta 1<br />Also known as: ATP1B<br />The protein encoded by this gene belongs to the Na+/K+ and H+/K+ atpase β-catenin family, and belongs to the Na+/K+-atpase subfamily. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradient of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, sodium-coupled transport of various organic and inorganic molecules, and electrical excitability in nerves and muscles. The enzyme is composed of two subunits, a larger catalytic subunit (α) and a smaller glycoprotein subunit (β). The β subunit regulates the number of sodium pumps delivered to the plasma membrane through the assembly of α/β heterodimers. The glycoprotein subunit of the Na+/K+-atpase is encoded by multiple genes. This gene encodes the β1 subunit. Alternative splicing transcript variants encoding different isoforms have been described, but their biological validity is unclear.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements