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Analysis of ATP2B4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0809-LGZ)

Introduction

Official Full Name: ATPase plasma membrane Ca2+ transporting 4<br />Also known as: MXRA1; PMCA4; ATP2B2; PMCA4b; PMCA4x<br />The protein encoded by this gene belongs to the P-type primary ion transporting ATPase family, which is characterized by the formation of an aspartate phosphate intermediate in the reaction cycle. These enzymes remove divalent calcium ions from eukaryotic cells in very large concentration gradients and play a key role in intracellular calcium homeostasis. Mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes, and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different alloforms and splice variants is regulated in a developmental, tissue and cell type specific manner, suggesting that these pumps are functionally adapted to the physiological demands of specific cells and tissues. This gene encodes plasma membrane calcium ATPase isoform 4. Alternative splicing transcript variants encoding different isoforms have been identified.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements