Analysis of ATP8B2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0807-LGZ)

Introduction

Official Full Name: ATPase phospholipid transporting 8B2<br />Also known as: ATPID<br />The protein encoded by this gene belongs to the p-type cation transporting ATPase family and belongs to the aminophospholipid transporting ATPase subfamily. Aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of the bilayer to the other. Alternative splicing transcript variants encoding different isoforms have been identified.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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