Analysis of B-cell Neoplasias by Spectral Karyotyping (SKY) (CAT#: STEM-ST-0383-WXH)

Introduction

B-cell neoplasias represent a heterogeneous group of diseases, including acute lymphocytic leukemia (ALL) and the broad spectrum of non-Hodgkin’s lymphomas (NHL). Conventional cytogenetic analysis has revealed specific chromosomal aberrations in ALL as well as in NHL. Spectral karyotyping (SKY) is a novel molecular cytogenetic technique which allows the visualization of all human chromosomes in different colors, therefore greatly facilitating the recognition of chromosomal aberrations.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

Other recommended products

Detection of chromosomal aberrations by Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0373-WXH)
Detection of unidentified chromosome abnormalities in human neuroblastoma by spectral karyotyping (SKY) (CAT#: STEM-ST-0384-WXH)
Spectral karyotype analyses of cartilage tumours (CAT#: STEM-ST-0381-WXH)
Study of primary breast carcer by Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0386-WXH)
Study on human hepatocellular carcinoma by spectral karyotyping (CAT#: STEM-ST-0374-WXH)
Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0370-WXH)
Karyotype analysis of human cytotrophoblasts (CAT#: STEM-ST-0385-WXH)
Characterization of chromosomal abnormalities in prostate cancer cell lines by spectral karyotyping (CAT#: STEM-ST-0378-WXH)