Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of B4GALT3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0804-LGZ)

Introduction

Official Full Name: beta-1,4-galactosyltransferase 3<br />Also known as: beta4Gal-T3<br />This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all galactose are transferred in a β1,4 bond to similar acceptor sugars: GlcNAc, Glc and Xyl. Each β-4galt has a different function in the biosynthesis of different glycoconjugates and sugar structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus, where it remains uncleaved and serves as a transmembrane anchor. According to sequence similarity, beta4GalTs are divided into four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactamine unit of the poly-N-acetyllactamine chain. Multiple alternatively spliced transcript variants of this gene encoding the same protein have been found.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements