Analysis of B7-H6 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0047-LGZ)

Introduction

B7-H6 is a ligand for the NK cell-activating receptor NKp30, which triggers cytotoxicity and cytokine secretion against tumor natural killer (NK) cells. Both forms of B7-H6 (cell surface B7-H6 and soluble B7-H6 (sB7-H6)) are detected in primary tumors and the tumor microenvironment, but are rarely found in normal tissues.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Immunology/Inflammation, Cytokines & Chemokines

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma

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