Analysis of Bacteria by Real-Time PCR Method (CAT#: STEM-MB-2807-LGZ)

Introduction

A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.

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Principle Applications Procedure Materials Notes Related Products

Principle

Bacteria, or prokaryotes, in a broad sense refer to a large group of primitive single-celled organisms whose nuclei have no nuclear envelope and only bare DNA known as a nuclear region (or archaea). They include eubacteria and Archaea. People usually refer to bacteria in a narrow sense. Bacteria in a narrow sense is a kind of prokaryotic microorganism, which is a kind of short shape, simple structure, and prokaryotic organisms that reproduce in the way of dimitosis. It is the most widely distributed organism in nature with the largest number of individuals, and the main participant in the material cycle of nature.
This experiment is based on RT-qPCR technology, and primers and probes have been optimized with high sensitivity. High specificity, primers are designed according to highly conserved areas of bacteria, and will not cross-react with other pathogen DNA.

Applications

Used to detect bacteria.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: serum

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