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Analysis of Bcl-2 (Human) by ELISA (CAT#: STEM-MB-0691-LGZ)

Introduction

Bcl-2 gene (namely B-cell lymphoma/leukemia-2 gene) is an oncogene, which can obviously inhibit cell apoptosis, and some studies in recent years have begun to reveal the mechanism of this effect. Bcl-2 is the main target molecule in the study of apoptosis molecular mechanism. With the deepening of the research on Bcl-2 and apoptosis itself, the mechanism of action of Bcl-2 and the molecular mechanism of apoptosis are finally elucidated, thereby improving the understanding, diagnosis and treatment of diseases related to apoptosis.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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