Analysis of Beta-carotene by UV-Vis Spectroscopy (CAT#: STEM-MB-0943-WXH)

Introduction

Beta-carotene is a pigment found in plants that gives them their color. The name beta-carotene is derived from the Latin name for carrot. It gives yellow and orange fruits and vegetables their rich hues. Beta-carotene is also used to color foods such as margarine.
Beta-carotene is an antioxidant. It protects the body from damaging molecules called free radicals. Free radicals damage cells through a process known as oxidation. Over time, this damage can lead to a number of chronic illnesses.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer

Other recommended products

Monitering the fermentation of Escherichia coli by UV-Vis Spectroscopy (CAT#: STEM-MB-0878-WXH)
Analysis of Interaction between Diosmetin and serum albumin by UV-Vis Spectroscopy (CAT#: STEM-MB-0934-WXH)
Monitoring of Transformations Between Nucleosides and Nucleobases by UV-Vis Spectroscopy (CAT#: STEM-MB-0965-WXH)
Quantitative investigation of platelet quality by UV-Vis Spectroscopy (CAT#: STEM-MB-0889-WXH)
Analysis of Silicone oil induced aggregation of proteins by UV-Vis Spectroscopy (CAT#: STEM-MB-0940-WXH)
Analysis of proteins in animal depression model investigated by spectroscopic techniques by UV-Vis Spectroscopy (CAT#: STEM-MB-0982-WXH)
Study on the interactions of porphyrin with G-quadruplex DNAs by UV-Vis Spectroscopy (CAT#: STEM-MB-0920-WXH)
Analysis of the interaction of gallic acid and myoglobin by UV-Vis Spectroscopy (CAT#: STEM-MB-0969-WXH)