Analysis of Betacellulin/BTC by ELISA (CAT#: STEM-MB-1561-LGZ)

Introduction

Betacellulin, also known as the BTC protein, is encoded in humans by the BTC gene in region 4q13.3 on chromosome 4. The protein encoded by the gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor and processed into mature molecules by proteolysis. This protein is a ligand of the EGF receptor and binds to EGFR, ERBB4, and other members of the EGF receptor family. It is also an effective mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Data suggest that BTC effectively promotes angiogenesis through EGF receptor activation in endothelial cells, mitogen-activated protein kinases, and phosphatidylinositol 3' kinase/protein kinase.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Diabetes Mellitus

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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