Analysis of BGLAP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0800-LGZ)

Introduction

Official Full Name: bone gamma-carboxyglutamate protein
Also known as: OC; BGP; OCN
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamic acid) domain that functions to bind calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be inversely correlated with metabolic syndrome. There is a read-through transcription between this gene and the adjacent upstream gene PMF1 (polyamine regulatory factor 1), but the encoded protein is only consistent with the upstream gene product sequence.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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