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Bioactives are chemical compounds often referred to as secondary metabolites. Thirty five bioactive compounds were identified in the methanolic extract of Aspergillus niger. Gas chromatography-mass spectrometry (GC-MS) analysis of Aspergillus niger revealed the existence of the 6-Acetyl-ß-d-mannose, 4-[Dichloromethyl]-2-[[2-[1-methyl-2-pyrrolidinyl]ethyl]amino-6-trichloro, 2-Furan-carboxaldehyde,5-methyl, 2,2,2-Trifluoro-N-[2-(1-hydroxy-2,2,6,6-tetramethyl-piperidin-4-yl), 2,4-Dihydroxy-2,5-dimethyl-3(2H)-furan-3-one, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), Tetraacetyl-d-xylonic nitrile, Eicosanoic acid, phenylmethyl ester, Dodecanoic acid, 3-hydroxy, Desulphosinigrin, Glycyl-dl-serine, 2,5-Dimethyl-4-hydroxy-3(2H)-furanone, 2,5-Furandicarboxaldehyde, 2H-Oxecin-2-one,3,4,7,8,9,10- hexahydro-4-hydroxy-10-methyl, 6-Acetyl-ß-d-mannose, DL-Leucine, N-glycyl, 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl, I-Gala-l-ido-octonic lactone, 2H-Pyran,tetrahydro-2-(12-pentadecynyloxy), 5-Hydroxymethylfurfural, Strychane,1-acetyl-20α-hydroxy-16-methylene, α-D-Glucopyranoside, O-α-D-glucopyranosyl-(1.fwdarw.3)ß-D-fru, Boroxin, tris(2,3-dimethylbut-2-yl), 16-Nitrobicyclo[10.4.0]hexadecane-1-ol-13-one, 3-[3-Bromophenyl]-7-chloro-3,4-dihydro-10-hydroxy-1,9(2H,10H)-a, Uric acid, 1,2,4-Trioxolane-2-octanoic acid ,5-octyl-,methyl ester, Tetraacetyl-d-xylonic nitrile, 1,2-Cyclopentanedicarboxylic acid, 4-(1,1-dimethylethyl)-,dimethyl, 2-Bromotetradecanoic acid, i-Propyl 11,12-methylene-octadecanoate, 1H-2,8a-Methanocyclopenta[a]cyclopropa [e]cyclodecan-11-one, and Octadecanoic acid.