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Analysis of BMP-7 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0064-LGZ)

Introduction

Bone morphogenetic protein (BMP), also known as bone morphogenic protein, is a group of highly conserved functional proteins with similar structures, belonging to the TGF-β family. BMP can stimulate DNA synthesis and cell replication, thereby promoting the directional differentiation of mesenchymal cells into osteoblasts. BMP-7 is the main interstitial factor, which can affect pathological processes such as fibrosis of interstitial components of alveolar tissue, increase airway hyperresponsiveness, and exacerbate airway smooth muscle spasm.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Bone Disorders

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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