Analysis of Bovine Papular Stomatitis Virus (BPSV) by Real-Time PCR Method (CAT#: STEM-MB-3033-LGZ)

Introduction

Bovine papular stomatitis virus (BPSV) causes mild disease in calves, characterised by flat to slightly raised red to brown macules and papules, sometimes progressing to ulcers, in the mucus membranes of the oral cavity. Diagnosis, treatment and prevention measures are rarely required, as severe disease is very rare.<br />A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.